Development of an improved isolation approach and simple sequence repeat markers to characterize Phytophthora capsici populations in irrigation ponds in southern Georgia.
Identifieur interne : 001B42 ( Main/Exploration ); précédent : 001B41; suivant : 001B43Development of an improved isolation approach and simple sequence repeat markers to characterize Phytophthora capsici populations in irrigation ponds in southern Georgia.
Auteurs : Ziying Wang [États-Unis] ; David B. Langston ; Alexander S. Csinos ; Ronald D. Gitaitis ; Ronald R. Walcott ; Pingsheng JiSource :
- Applied and environmental microbiology [ 1098-5336 ] ; 2009.
Descripteurs français
- KwdFr :
- Alanine (analogues et dérivés), Alanine (pharmacologie), Amorces ADN (génétique), Analyse de regroupements (MeSH), Anti-infectieux (pharmacologie), Génotype (MeSH), Géorgie (MeSH), Maladies des plantes (microbiologie), Microbiologie de l'eau (MeSH), Phytophthora (classification), Phytophthora (génétique), Phytophthora (isolement et purification), Profilage d'ADN (méthodes), Pyrus (microbiologie), Solanum melongena (microbiologie), Séquences répétées d'acides nucléiques (MeSH), Épidémiologie moléculaire (MeSH).
- MESH :
- analogues et dérivés : Alanine.
- génétique : Amorces ADN, Phytophthora.
- isolement et purification : Phytophthora.
- microbiologie : Maladies des plantes, Pyrus, Solanum melongena.
- méthodes : Profilage d'ADN.
- pharmacologie : Alanine, Anti-infectieux.
- Analyse de regroupements, Génotype, Géorgie, Microbiologie de l'eau, Séquences répétées d'acides nucléiques, Épidémiologie moléculaire.
English descriptors
- KwdEn :
- Alanine (analogs & derivatives), Alanine (pharmacology), Anti-Infective Agents (pharmacology), Cluster Analysis (MeSH), DNA Fingerprinting (methods), DNA Primers (genetics), Genotype (MeSH), Georgia (MeSH), Molecular Epidemiology (MeSH), Phytophthora (classification), Phytophthora (genetics), Phytophthora (isolation & purification), Plant Diseases (microbiology), Pyrus (microbiology), Repetitive Sequences, Nucleic Acid (MeSH), Solanum melongena (microbiology), Water Microbiology (MeSH).
- MESH :
- chemical , analogs & derivatives : Alanine.
- chemical , genetics : DNA Primers.
- chemical , pharmacology : Alanine, Anti-Infective Agents.
- geographic : Georgia.
- classification : Phytophthora.
- genetics : Phytophthora.
- isolation & purification : Phytophthora.
- methods : DNA Fingerprinting.
- microbiology : Plant Diseases, Pyrus, Solanum melongena.
- Cluster Analysis, Genotype, Molecular Epidemiology, Repetitive Sequences, Nucleic Acid, Water Microbiology.
Abstract
Phytophthora capsici, the causal agent of Phytophthora blight, is a major concern in vegetable production in Georgia and many other states in the United States. Contamination of irrigation water sources by P. capsici may be an important source of inoculum for the pathogen. A simple method was developed in this study to improve the efficiency of recovering P. capsici from fruits used as baits in irrigation ponds. In contrast to direct isolation on agar plates, infected fruit tissues were used to inoculate stems of pepper seedlings, and the infected pepper stems were used for isolation on agar plates. With isolation through inoculation of pepper stems, the frequency of recovering P. capsici from infected eggplant and pear fruits increased from 13.9% to 77.7% and 8.1% to 53.5%, respectively, compared with direct isolation on agar plates. P. capsici was isolated from seven out of nine irrigation ponds evaluated, with most of the ponds containing both A1 and A2 mating types and a 4:5 ratio of A1 to A2 when isolates from all ponds were calculated. All P. capsici isolates were pathogenic on squash plants, and only a small proportion (8.2%) of the isolates were resistant or intermediately sensitive to mefenoxam. Simple sequence repeats (SSRs) were identified through bioinformatics mining of 55,848 publicly available expressed sequence tags of P. capsici in dbEST GenBank. Thirty-one pairs of SSR primers were designed, and SSR analysis indicated that the 61 P. capsici isolates from irrigation ponds were genetically distinct. Cluster analysis separated the isolates into five genetic clusters with no more than two genetic groups in one pond, indicating relatively low P. capsici genetic diversity in each pond. The isolation method and SSR markers developed for P. capsici in this study could contribute to a more comprehensive understanding of the genetic diversity of this important pathogen.
DOI: 10.1128/AEM.00620-09
PubMed: 19581483
PubMed Central: PMC2737936
Affiliations:
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Langston</name></author><author><name sortKey="Csinos, Alexander S" sort="Csinos, Alexander S" uniqKey="Csinos A" first="Alexander S" last="Csinos">Alexander S. Csinos</name></author><author><name sortKey="Gitaitis, Ronald D" sort="Gitaitis, Ronald D" uniqKey="Gitaitis R" first="Ronald D" last="Gitaitis">Ronald D. Gitaitis</name></author><author><name sortKey="Walcott, Ronald R" sort="Walcott, Ronald R" uniqKey="Walcott R" first="Ronald R" last="Walcott">Ronald R. 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Contamination of irrigation water sources by P. capsici may be an important source of inoculum for the pathogen. A simple method was developed in this study to improve the efficiency of recovering P. capsici from fruits used as baits in irrigation ponds. In contrast to direct isolation on agar plates, infected fruit tissues were used to inoculate stems of pepper seedlings, and the infected pepper stems were used for isolation on agar plates. With isolation through inoculation of pepper stems, the frequency of recovering P. capsici from infected eggplant and pear fruits increased from 13.9% to 77.7% and 8.1% to 53.5%, respectively, compared with direct isolation on agar plates. P. capsici was isolated from seven out of nine irrigation ponds evaluated, with most of the ponds containing both A1 and A2 mating types and a 4:5 ratio of A1 to A2 when isolates from all ponds were calculated. All P. capsici isolates were pathogenic on squash plants, and only a small proportion (8.2%) of the isolates were resistant or intermediately sensitive to mefenoxam. Simple sequence repeats (SSRs) were identified through bioinformatics mining of 55,848 publicly available expressed sequence tags of P. capsici in dbEST GenBank. Thirty-one pairs of SSR primers were designed, and SSR analysis indicated that the 61 P. capsici isolates from irrigation ponds were genetically distinct. Cluster analysis separated the isolates into five genetic clusters with no more than two genetic groups in one pond, indicating relatively low P. capsici genetic diversity in each pond. The isolation method and SSR markers developed for P. capsici in this study could contribute to a more comprehensive understanding of the genetic diversity of this important pathogen.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19581483</PMID><DateCompleted><Year>2009</Year><Month>10</Month><Day>22</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1098-5336</ISSN><JournalIssue CitedMedium="Internet"><Volume>75</Volume><Issue>17</Issue><PubDate><Year>2009</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Applied and environmental microbiology</Title><ISOAbbreviation>Appl Environ Microbiol</ISOAbbreviation></Journal><ArticleTitle>Development of an improved isolation approach and simple sequence repeat markers to characterize Phytophthora capsici populations in irrigation ponds in southern Georgia.</ArticleTitle><Pagination><MedlinePgn>5467-73</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1128/AEM.00620-09</ELocationID><Abstract><AbstractText>Phytophthora capsici, the causal agent of Phytophthora blight, is a major concern in vegetable production in Georgia and many other states in the United States. Contamination of irrigation water sources by P. capsici may be an important source of inoculum for the pathogen. A simple method was developed in this study to improve the efficiency of recovering P. capsici from fruits used as baits in irrigation ponds. In contrast to direct isolation on agar plates, infected fruit tissues were used to inoculate stems of pepper seedlings, and the infected pepper stems were used for isolation on agar plates. With isolation through inoculation of pepper stems, the frequency of recovering P. capsici from infected eggplant and pear fruits increased from 13.9% to 77.7% and 8.1% to 53.5%, respectively, compared with direct isolation on agar plates. P. capsici was isolated from seven out of nine irrigation ponds evaluated, with most of the ponds containing both A1 and A2 mating types and a 4:5 ratio of A1 to A2 when isolates from all ponds were calculated. All P. capsici isolates were pathogenic on squash plants, and only a small proportion (8.2%) of the isolates were resistant or intermediately sensitive to mefenoxam. Simple sequence repeats (SSRs) were identified through bioinformatics mining of 55,848 publicly available expressed sequence tags of P. capsici in dbEST GenBank. Thirty-one pairs of SSR primers were designed, and SSR analysis indicated that the 61 P. capsici isolates from irrigation ponds were genetically distinct. Cluster analysis separated the isolates into five genetic clusters with no more than two genetic groups in one pond, indicating relatively low P. capsici genetic diversity in each pond. The isolation method and SSR markers developed for P. capsici in this study could contribute to a more comprehensive understanding of the genetic diversity of this important pathogen.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Ziying</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Coastal Plain Experiment Station, University of Georgia, Tifton, GA 31794, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Langston</LastName><ForeName>David B</ForeName><Initials>DB</Initials></Author><Author ValidYN="Y"><LastName>Csinos</LastName><ForeName>Alexander S</ForeName><Initials>AS</Initials></Author><Author ValidYN="Y"><LastName>Gitaitis</LastName><ForeName>Ronald D</ForeName><Initials>RD</Initials></Author><Author ValidYN="Y"><LastName>Walcott</LastName><ForeName>Ronald R</ForeName><Initials>RR</Initials></Author><Author ValidYN="Y"><LastName>Ji</LastName><ForeName>Pingsheng</ForeName><Initials>P</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>07</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Appl Environ Microbiol</MedlineTA><NlmUniqueID>7605801</NlmUniqueID><ISSNLinking>0099-2240</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000890">Anti-Infective Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>F1Q54ONN4H</RegistryNumber><NameOfSubstance UI="C473256">mefenoxam</NameOfSubstance></Chemical><Chemical><RegistryNumber>OF5P57N2ZX</RegistryNumber><NameOfSubstance UI="D000409">Alanine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000409" MajorTopicYN="N">Alanine</DescriptorName><QualifierName UI="Q000031" MajorTopicYN="N">analogs & derivatives</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000890" MajorTopicYN="N">Anti-Infective Agents</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016000" MajorTopicYN="N">Cluster Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016172" MajorTopicYN="N">DNA Fingerprinting</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005838" MajorTopicYN="N">Genotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005845" MajorTopicYN="N" Type="Geographic">Georgia</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017720" MajorTopicYN="N">Molecular Epidemiology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D031989" MajorTopicYN="N">Pyrus</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012091" MajorTopicYN="Y">Repetitive Sequences, Nucleic Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032323" MajorTopicYN="N">Solanum melongena</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014871" MajorTopicYN="Y">Water Microbiology</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>7</Month><Day>8</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>7</Month><Day>8</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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Csinos</name><name sortKey="Gitaitis, Ronald D" sort="Gitaitis, Ronald D" uniqKey="Gitaitis R" first="Ronald D" last="Gitaitis">Ronald D. Gitaitis</name><name sortKey="Ji, Pingsheng" sort="Ji, Pingsheng" uniqKey="Ji P" first="Pingsheng" last="Ji">Pingsheng Ji</name><name sortKey="Langston, David B" sort="Langston, David B" uniqKey="Langston D" first="David B" last="Langston">David B. Langston</name><name sortKey="Walcott, Ronald R" sort="Walcott, Ronald R" uniqKey="Walcott R" first="Ronald R" last="Walcott">Ronald R. Walcott</name></noCountry><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Wang, Ziying" sort="Wang, Ziying" uniqKey="Wang Z" first="Ziying" last="Wang">Ziying Wang</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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